Synergestic role of selenium and vitamin e in ameliorating the cigarette smoke induced biochemical alterations in albino rat

Present study was undertaken to investigate the synergistic role of selenium and vitamin E in ameliorating the cigarette smoke induced biochemical alterations in albino rats. A total of thirty six (36) adult albino rats of the Wister strain were used, they were randomly divided into three sets of twelve rats each. Set A served as control (no exposure to cigarette smoke). While rats in set B were exposed to only cigarette smoke and rats in set C were exposed to cigarette smoke with pre-supplementation of selenium and vitamin E. The rats were subjected to whole body exposure of cigarette smoke of a filtered cigarette 1hr/day (delivered as 6 cigarettes, 6 times/day) for 28 days. At the end of each experimental period, blood was obtained from each rat for the determination of serum total protein, serum urea, serum creatinine and serum glucose. An increase in serum urea, serum creatinine and serum glucose levels was observed, however serum total protein showed a considerable decrease in cigarette smoked albino rats. Due to the synergistic effect of Selenium and Vitamin E, the intensity of inflammation caused by cigarette smoke was reduced by antioxidant defence mechanism and the said altered biochemical parameter levels were considerably restricted to their normal ranges.