Production and characterization of biosurfactant by bacillus subtilis isolated from soil samples
International Journal of Development Research
Production and characterization of biosurfactant by bacillus subtilis isolated from soil samples
Received 28th May, 2017; Received in revised form 19th June, 2017; Accepted 20th July, 2017; Published online 30th August, 2017
Copyright ©2017, Sanjana S. Varma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study investigated the capability of a biosurfactant produced by a novel strain of Bacillus subtilis to enhance the biodegradation rates and bioavailability of organic contaminants isolated from soil sample. 10 isolates of Bacillus subtilis were isolated from three different oil contaminated soil samples. All isolates showed α – haemolysis in haemolytic test. In oil displacement test, isolates BS1, BS2, BS3, BS4, BS5, BS7 and BS8 showed zone of diameter 25mm, 13mm, 18mm, 16mm, 9mm, 11mm and 18mm respectively. The E24 % of BS1, BS2, BS3, BS4, BS5, BS7 and BS8 were 38.46%, 15.38%, 35.71%, 30.76%, 15.38%, 14.28% and 23.07% respectively. Drop collapse test were given positive by isolates BS1, BS2, BS3, BS4, BS7 and BS8. The emulsification activity of produced biosurfactant is calculated by emulsification index (E24). E24% of biosurfactant produced by BS1, BS2, BS3, BS4 and BS8 are 34.21%, 19.44%, 32.43%, 31.57% and 16.66% respectively. BS1, BS2 and BS4 showed maximum biosurfactant activity and highest dry weight found in isolate BS1 (0.05 gm/100ml). Overall, the results indicated the potential use of B. subtilis in environmental remediation processes.
