Preliminary qualitative phytochemical, physicochemical and biochemical Charecterization of herbal formulation nochiver kudineer (Vitex negundo)

Introduction: The Siddha system is one of the ancient system of medicine created by siddhars. Thandagavatham is one among the vatha disease, it was coming under the head of degenerative disease. The clinical symptoms and sign is similarly in correlated in modern science is lumbar spondylosis. Thandagavatham is mentioned in various text books and manuscript. Among the internal medicines Nochi ver kudineer (Vitex negundo. Verbenaceae family) is indicated for Thandagavatham (Lumbar spondylosis) in Gunapadam part- 1 (Mooligai vaguppu). In Indian traditional medicine system vitexnegundo Linn is referred as “ Sarvaloganivarani” – the remedy for all disease. Vitex negundo Root mainly deals with Bronchitis,Asthma, Dysentery, Irritable bowel, Fever, Urinary problem, Calculus, Gout, Rheumatism by its various mode of pharmacological properties like Analgesic, Anti inflammatory, Anti oxidant, Anti pyretic, Arthritic, Anti microbial, Nephro protective activity, Antinociceptive activity. Aim: The aim of the study is to perform qualitative Phytochemical evaluation, physicochemical analysis, Biochemical analysis of Nochi ver kudineer. Material and methods: The raw drug was purchased from reputed herbal drug shop, Thackkalay, kanyakumari district and it is purified then made into decoction as per the siddha literature. Results: The Phytochemical evaluation showed the presence of Terpenoids, Phenols, Quinones, Glycosides, Alkaloids (Table 2). The Bio chemical analysis showed the presence of Sulphate, Chloride, Ferrous iron, Tannic acid, Unsaturated compounds, Reducing sugar and Aminoacid (Table 4). The Physico chemical analysis showed the total ash 2.61% Acid insoluble ash 0.35%, Water soluble ash 0.86%, Sulphate ash 4.44%, Alcohol soluble extractives 6.66%, Water soluble extractives 5.96% (Table 3) were observed from the study.