Phytochemical screening and in vitro evaluation of cytotoxic activity of fruits of meyna spinosa roxb

Meyna spinosa Roxb, a medicinal plant enjoys it use in the traditional medicine in all over the India for the treatment of a number of ailments. In Bengali it is called as Monkata. It is a wild common plant distributed in India, Bangladesh and in both northeastern tropical Africa and tropical Asia. The main objective of the present research work was to determine various bioactive compounds especially phenolic and flavonoid contents, carbohydrates, phytosterols, aminoacids and proteins and to evaluate the in vitro cytotoxic activity of different fruit extracts of Meyna spinosa Roxb . Based on this, a new series of constituents had been planned to extract by Methanol (ML), Ethanol (EL), and Chloroform (CF) from the fruits of Meyna spinosa Roxb. The in-vitro cytotoxic activity was carried out against Human Prostate cancer cell line DU-145 and SRB assay was used to analyze the cell growth inhibition of the cancer cell line. 5-Flurouracil (5-FU, at 10µg/ml) was used as a standard drug for DU-145 cell line. The results showed that the various extracts of fruits of Meyna spinosa Roxb, had a potential cytotoxic  activity against DU-145 cell lines. The IC50 values of the various extracts of fruits of Meyna spinosa Roxb were found to be ML (IC50 of   2.5 µg/ml), EL (IC50 of 2.3 µg/ml), and CF (IC50 of 2.1 µg/ml) for DU-145   cell line.