Performance evaluation of a molecular method to simultaneously detect Salmonella Enteritidis/Salmonella Typhimurium in a chicken meat matrix instead of conventional serology
International Journal of Development Research
Performance evaluation of a molecular method to simultaneously detect Salmonella Enteritidis/Salmonella Typhimurium in a chicken meat matrix instead of conventional serology
Received 10th August, 2021; Received in revised form 24th August, 2021; Accepted 14th September, 2021; Published online 30th September, 2021
Copyright © 2021, Mariana Dammann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) are the major agents involved in food outbreaks worldwide. Developing quick tests to better detect this pathogen in food is crucial to ensure safety and meet the growing demand for animal products. Therefore, this study aimed to evaluate the performance of a molecular method to simultaneously detect SE/ST in a chicken meat matrix instead of conventional serology. For this, each pathogen-free sample was contaminated, being single or combined (SE, ST, Salmonella spp.). Protocols for NewGene FastX extraction, simultaneous qPCR SETAmp detection, and Salmonella SERef and STRef positive controls were followed, and out of eight samples analyzed,five presented 100% agreement with the expected result.The samples were also tested with Escherichia coli, Klebsiella aerogenes, and Citrobacter freundii, which did not amplify, thereby confirming the kit’s specificity. There are several kits for simultaneous detection marketed by international companies, although this study is one of the first to validate a Brazilian produced and marketed kit. This is an alternative diagnostic procedure that may replace the serology stage of the conventional Salmonella spp. method.
