Organogenesis induction in foliar explants and nodal segments of solanum paniculatum l
International Journal of Development Research
Organogenesis induction in foliar explants and nodal segments of solanum paniculatum l
Received 25th January, 2021 Received in revised form 17th January, 2021 Accepted 20th February, 2021 Published online 30th March, 2021
Copyright © 2021, Faustino S. Luciely et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Solanum paniculatum L. popularly known as jurubeba, is a shrub that has pharmacological importance. In this way, micropropagation emerges as an alternative to enable the production of seedlings on a large scale, presenting several advantages from the multiplication of genetic material, for the exchange or evaluation of germplasms, to the production of virus free seedlings. The aim of this study was to perform an efficient in vitro establishment of organogenesis from leaf explants and nodal segments of jurubeba under the effect of different concentrations of 6-benzylaminopurine (BAP) and naphthalene-acetic acid (NAA), (BAP: 0.0, 1.0, 2.0, 4.0 or 8.0 mg L-1; NAA: (0.0, 0.5 or 1.0 mg L-1). The experiment was carried out in DIC (Entirely Randomized Design), and elaborated in a 3x5 factorial scheme, showing statistically significant differences (p <0.05) for all variables analyzed. To induce greater callus formation in leaf explants and nodal segments, the use of BAP and NAA (1.0 mg L-1) is recommended. For bud formation, from leaf explants, it is recommended to promote the use of 1.0 mg L-1 NAA without using BAP. For nodal segments, a greater number of shoots is obtained with 8.0 mg L-1 of BAP without using the NAA growth regulator.