Epstein-Barr Virus Infection And Polymorphisms In Human Leukocyte Antigen Genesin Transplant Donors And Receptors From The State Of Pará, Amazon, Brazil

Background: This work aimed to perform the molecular detection of EBV in samples from donors and recipients of living donor kidney transplantations at the HEMOPA Foundation, in Pará state, an Amazon region, and to characterize the prevalence of EBV and allele frequency of HLA in this population, and seek associations between these two factors. Methods: EBV detection was performed on 200samples of candidate donors and recipients using Nested PCR. We collected HLA classification results from these same individuals and 200 bone marrow donors as the control group in an HLA allelic frequency analysis database. Results: The EBV detection rate was similar between candidate donors and recipients. Among the 73 analyzed pairs of donors and recipients, 12 consisted of an undetectable recipient and a detectable donor. The B*41 and B*37 alleles were more frequent in the EBV+ group of patients than the EBV- group, unlike the DRB1 * 08 allele, which was more frequent in the EBV- group. Among EBV+ patients and controls, the A*01 allele was more frequent among detectable patients, while the DRB1*08 and DRB1*10 alleles were more frequent in the control group. The B*08 and DRB1*3 alleles were more frequent in the control group than EBV- patients. Conclusion: The significance of these results was shown in Fisher's Exact Testor Chi-Square test; however, no significance was observed after Bonferroni's correction. Increased sample sizes may provide more robust validation of these results in the future.