Molecular study based on proteus mirabilis contamination in chicken meat in selected districts of Kerala, India

Proteus mirabilis is known human pathogen as a common cause of human urinary tract infection (UTI), nosocomial infection, wound infection and a possible causative agent of outbreaks of gastroenteritis, resulting from the consumption of contaminated food. In this study, we have analyzed bacteria in the chicken meat in selected districts of Kerala, India, using 16S rRNA gene partial sequencing.. 50 Samples of raw chicken meat were collected from slaughterhouses and meat shops in the study area by simple random sampling method. Bacteriological analysis was carried out and the black colonies appeared on the plates were selected and drew out for further confirmation by PCR analysis. The PCR product was sequenced using Sanger’s method. Then the trimmed forward and reverse sequences were assembled by using Clustal Omega and consensus sequence was taken for analysis by nucleotide BLAST programme. By examining 50 samples, it was confirmed that 1 sample from Kottapuram (Malappuram district) was contaminated by P. mirabilis. Maintaining a proper hygienic environment is an important step to avoid P. mirabilis related health hazards in humans or spreading to other birds. Microbial control in each and every stage of chicken production can control an outbreak of P. mirabilis contamination in chicken meat.