Investigations on biochemical components of aloe ferox mill leaf extracts and their antioxidant and anti-inflammatory properties
International Journal of Development Research
Investigations on biochemical components of aloe ferox mill leaf extracts and their antioxidant and anti-inflammatory properties
Received 19th November, 2017; Received in revised form 25th December, 2017; Accepted 24th January, 2018; Published online 28th February, 2018
Copyright © 2018, Chandra Sekhar Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present investigations were carried out to understand the phytochemical screening, antioxidant, anti inflammatory and biochemical properties of Aloe ferox leaf extract by using various organic solvents. The powdered leaf material was subjected to extraction of phytochemicals by soxhlet method with chloroform, ethyl acetate and methanol. The phytochemical result shows that the active compounds presence in high concentration, such as phytosterols, lactones, flavonoids, phenolic compounds, tannins and glycosides. This methanolic extract of Aloe ferox shows promising antioxidant DPPH radicals decreased in DPPH free radical scavenging assay and these extracts also shows more flavonoids than the other solvent extracts of Aloe ferox leaf, which also shows the reductive capabilities when compared with BHT. The methnol extract of Aloe ferox (AFME) shows significant inhibition against carrageenan-induced paw edema in the dose dependent method. Aloe ferox exhibits highest anti-inflammatory and analgesic activities in the highest dose (500 mg/kg) tested. This dose level exerted highest anti-inflammatory activity of 0.61% and 0.52% for carrageenan induced paw edema respectively. The analgesic activity was observed as 38% and 50% for the 250/500 mg/kg dose in phase 3 and 4, respectively and increase in the paw volume. The anti-inflammatory activity exerted by AFME suggests that it could affect by bradykinin, kinnin, prostaglandin and synthesis of lysozymes. The methanol leaf extracts were structural elucidation and isolated an anticancer compound of acemannan were analyzed by HPLC and 13C NMR at the mobile phase of methanol: acetnitrile: water (in the ratio of 25:35:40) and provided a total of 6 peaks at a retention time 4.545 min. From the HPLC results, the highest peak was observed at retention of 563.778 mAU. 13C-NMR results show a number of peaks in between δ 16.832 to 71.196 and δ 174.6 to 176.777 (indicating presence of aromatic rings), the highest peak was observed between 48.996 - 49.007.
