Antibacterial activity of excoecaria agallocha l.leaf in chloroform and ethanol extracts (gc-ms analysis)

The present study was carried out for Antibacterial Activity and GC-MS analysis of Excoecaria agallocha L. leaf extracts of Chloroform and Ethanol. Antibacterial assay was carried out against five bacteria viz. Staphylococcus aureus MTCC3381, Bacillus cereus MTCC430, Escherichia coli MTCC739, Pseudomonas aeruginosa MTCC 424, Klebsiella pneumoniae MTCC432 using agar well diffusion method. Chloroform extract of E.agallocha was highly effective on Bacillus cereus strain. There was no effect on E.coli and K.pneumoniae. It was moderately effective in 2000µg concentration only. P.aeruginosa was affected moderately in 1500 and 2000µg concentrations. Ethanol extract was highly effective than Chloroform extract. Ethanol extract was ineffective in 500µg on E.coli, Bacillus cereus, Pseudomonas aeruginosa and Klebsiella pneumoniae. In 1500µg Ethanol extract was ineffective on P.aeruginosa. Ethanol extract was ineffective in 1000µg concentration on K.pneumoniae. It was highly effective on S.aureus at 500,1000,1500,2000µgs. GC-MS analysis was tested in chloroform and ethanol extracts of E.agallocha L. In Chloroform extract 34 bio-active compounds were identied. From that Hentriacontane 5.23%, Tricyclo, undec-tetramethyl 16.38%, Tetramethyl, trihydro-napthalene 20.72%, β - amyrin 7.52%, α – amyrin 6.92%, Heptadecanol 8.24% were in high proportions. Ethanol extract showed 17 bio-active compounds, in that Myoinosital 4-c-methyl 37.09%, Tricyclo undec (isocaryophyllene) 13.21%, Trimethyl 5,6-dimethylene-deca hydro naphthalene 17.25%, β- amyrin 5.78%, α- amyrin 5.78% were observed as the major constituents.