Measurement and feasibility of hematopoietic stem cell was greater for equipment in closed system

Worldwide are held about 16,000 transplants of stem cells autologous and 29,000 allogeneic. In Brazil, the stem cell transplant autologous bone marrow transplantation increases about 1,000 per year. Cell therapy or transplantation of Hematopoietic Stem Cells (HSC) is a regenerative medicine that aims to restore and regenerate the functions of senescent cells or tissues with serious injuries. The CTH constitute from 0.05% to 0.1% of human bone marrow and circulating haematopoietic cells. The phenotype of hematopoietic stem cells include CD 34 antigen expression of the (+) and CD133 (+). Samples were collected in 120 ml         (± 5.0 %) blood from bone  marrow, from a universe sample consisted of fifteen patients  and  the  comparison  between  the  manual  method  and  system SEPAX.  For all samples  the quantification of total leukocytes (CD34 (-)) ranged from 3.5 x  103 to 6.5 x103, no evidence of infection. The average measurements of cells extracted by SEPAX were statistically superior to the manual method and the technique SEPAX obtained a lower standard deviation of measurements, showing greater accuracy of the technique. Furthermore, there was no statistically significant correlation (p-value > 0.05) among these techniques, proving that the method can be SEPAX greater control of the results. Added to this, the technique SEPAX, achieved a higher viability of hematopoietic stem cells. Therefore, the system opens SEPAX advantages over manual by several factors, as well as ensuring the sterility of the product also allows extracting higher number of cells CD34 (+), with greater viability.