Isolation and identification of a novel Thermoalkalophilic isolate bacillus Aerius gc6 for amylase production under submerged fermentation

A potential amylase producing bacterial strain was isolated from mushroom compost and identified as Bacillus aerius GC6 using 16S rRNA gene technique and deposited in NCBI gene bank vide accession number |KJ775810.1|. The aim of this study was to enhance amylase production by optimizing various medium components and process parameters viz. substrate concentration, pH, temperature, inoculums size, Ca2+ and Mg2+ concentrations through the statistical approach under submerged fermentation from this isolate. The optimum levels of these six parameters and their interaction effects were determined employing the response surface central composite design (CCD). A maximum amylase activity of 84.645 IU/ml had been obtained at 47.5 °C and 9.5 pH @ 8.75% inoculum using 1.75 % substrate with 0.5% Ca2+ and Mg2+ concentrations. An overall increase of 13.11% has been observed after the factors were optimized by using RSM.