Protein pattern in chlorpyrifos treated zebrafish embryos

Chlorpyrifos a broad spectrum product has been in use for many applications in home and agriculture against the damages caused by pests around the globe. This extreme usage has lead to detrimental effects on biological life. The goal of the present study was to determine the toxic effects of chlorpyrifos during early development in zebrafish. The embryo/larvae were exposed to 200μg/L, 400μg/L, 600μg/L, 800μg/L and 1000μg/L of CP and observations were made at 24hpf, 48hpf, 72hpf and 96hpf. Changes in the protein content was estimated and proteins were resolved by SDS-PAGE. The results indicate that chlorpyrifos evoked alterations in the protein content during development. SDS-PAGE gels have revealed seven bands at molecular weight marker at 205kDa, 97kDa and three bands between 43-29kDa, and 20.1kDa showed increase/decrease in their intensity at different developmental stages. These bands comprise of detoxifying enzymes, vitellogenins, nuclear, membrane, centrosomal, mitochondrial, cytosolic and heat shock proteins. From these results it can be said the CP causes modulations in protein profile.