New validated rp-hplc method for the estimation of sumatriptan succinate in pharmaceutical formulation
International Journal of Development Research
New validated rp-hplc method for the estimation of sumatriptan succinate in pharmaceutical formulation
Received 29th March, 2017; Received in revised form; 14th April, 2017; Accepted 06th May, 2017; Published online 30th June, 2017
Copyright ©2017, Sivanadh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Introduction: A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Sumatriptan Succinate in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1.5 ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column Column at ambient temperature. The mobile phase consisted of Orthophosphoric acid(1%):Acetonitrile : Methanol (90:5:5 v/v) . The UV detection wavelength was 210nm and 20 µl sample was injected. The run time for Sumatriptan Succinate is 6 min. The Percentage assay of Silymarin in formulation was found to be 100.08%. The amount of drug present in the human sample was found to be 0.287 mg/ ml The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Sumatriptan Succinate formulations.
