Isolation of protein from cell free culture filtrate: A noble approach

Bacteria are well known for their ability to liberate proteins and enzymes into their environment. Pseudomonad sp. being important micro-organisms produces wide range of extra-cellular enzymes including proteases. The pseudomonad strains R62 and R81 has been used in this study for electrophoresis analysis of extracellular protease enzymes which were present in the cell free culture of nutrient broth (NB) and protease specific broth (PB) media. The SDS-PAGE analysis of the crude protein from cell free culture filtrate from both the media when analyzed for proteases, showed the presence of different bands in the electrophoretic field.