Holocellulolytic hydrolases production by filamentous fungi using oil cakes as substrate

This work evaluated the use of cottonseed, sunflower and macauba oilcakes as sources of carbon for the production of cellulolytic and xylanonytic enzymes using submerged fermentation. Aspergillus tubingensis AN1257 e Penicilliumsp T1.1, isolated from environmental samples, were tested. Trichoderma reesei CCT2768 was used as a referential lineage. The cottonseed oilcake distinguished itself because it provided the highest endoglucanase production with the all evaluated fungi. The enzymatic extract obtained from the A. tubingensis AN1257 cultivated with cottonseed oilcakes exhibited values of 0.694 U mL-1 for endoglucanase, 0.620 U mL-1 for β-glucosidase, 0.048 U mL-1 for FPase after 96 hours of fermentation, and 37 U mL-1 for xylanase after 72 hours of fermentation. The production of endoglucanase by the A. tubingensis AN1257, using cottonseed oil cakes, was 13.6% higher than the amount produced by T. reesei CCT2768. These preliminary results point to the promising use of isolated A. tubingensis lineages and cottonseed oil cakes for the production of cellulases and xylanese.