Genetic diversity analysis of butea monosperma from different agroecological regions of india using issr marker system and its comparison with RAPD

In the present paper, we report the use of ISSR markers and their comparison with RAPD to analyze the genetic diversity of Butea monosperma from different agroecological regions viz. arid, semi-arid, dry sub-humid and moist sub-humid. For this purpose, 26 ISSR primers were tested. Of them 7 reproducible primers yield 58 (83%) polymorphic bands. The average value of Nei’s gene diversity and resolving power were 0.71 and 7.47 respectively. The UPGMA based dendogram clustered all individuals into three distinct clusters with one sample from Bangalore placed outside the major cluster and at one end of the dendogram. The coefficient of genetic differentiation (GST) shows the lesser genetic variation among population and within population then from RAPD. The estimated value of correlation coefficient ie 0.84 indicated a good correlation and shows that these two methods are informative and could accurately detect the structure of genetic diversity and its partition within and between groups.