Effects of Adding Curcumin on Quality of Post-Thawed Arabian Stallion Semen

The objective of this study was to investigate the impact of including curcumin antioxidant on the viability of sperm after the processes of cooling and freezing. curcumin antioxidant was used in HF-20 extender at concentrations of 0.32, 0.60, 1.30, and 2.71 µm/L. HF-20 extender was a basic extender and used for control group. The post-thawed semen exhibited significantly higher total motility in the 0.32 curcumin treatment groups as compared to others. The extender containing 0.32 µm/L curcumin showed a significantly higher in total motility compared to others. Progressive motility values were similar among extenders. Speed motion of stallion sperm in extender containing curcumin showed significantly higher on VCL, VSL, VAP, LIN and STR compared to control. The findings of the plasma membrane integrity (HOST obtained with curcumin extenders at 0.32, 0.60, 1.30 µm/L were significantly high compared to control extender. Normal morphology and vital test sperm were significantly higher in control compared to curcumin extenders. Acrosome integrity was not significant difference among group. However, significantly higher DNA fragmentation was showed in curcumin 0.32 µm/L and control extenders. In conclusion, curcumin antioxidant has shown the capacity to enhance the quality of frozen-thawed sperm. On other hand, negatively influence on the viability and integrity of DNA inside the frozen-thawed spermatozoa. Therefore, more research to find the optimal level of curcumin andadditional research should be conducted to assess the fertility potential of cryopreserved stallion semen.