Detection of brucella spp. in blood samples of water buffalo (bubalus bubalis) from marajó island, Amazon, Brazil
International Journal of Development Research
Detection of brucella spp. in blood samples of water buffalo (bubalus bubalis) from marajó island, Amazon, Brazil
Received 20th July, 2020; Received in revised form 19th August, 2020; Accepted 16th September, 2020; Published online 30th October, 2020
Copyright © 2020, Jacqueline C. Costa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Brucellosis is a global zoonotic infectious disease with great public health and economical importance, which affects the buffalo herds worldwide. Diagnostic methods for brucellosis usually depend on serological tests and direct culture, which may lack sensitivity. Thus, we aim to detect Brucella spp. using the conventional polymerase chain reaction (PCR) method in blood samples water buffaloes from five municipalities on Marajó Island, Eastern Amazon. A total of 97 male buffalos were sampled during slaughter and blood submitted to PCR. Among the 97 samples analyzed, 33% were positive and 36% were from Chaves, 27% from Cachoeira do Arari, 18% from Soure, 12% from Santa Cruz do Arari and 3% from the municipality from Salvaterra. It appears that Brucella spp. it is highly prevalent in the blood of male domestic water buffaloes from Marajó Island.
