Detection all four serotypes of dengue virus in aedes aegypti mosquitoes captured in tres lagoas – MS, Brazil
International Journal of Development Research
Detection all four serotypes of dengue virus in aedes aegypti mosquitoes captured in tres lagoas – MS, Brazil
Received 27th June 2020; Received in revised form 13th July 2020; Accepted 04th August 2020; Published online 30th September 2020
Copyright © 2020, Rachid Figueirôa Souza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Três Lagoas - MS has favorable characteristics for the reproduction and maintenance of the Dengue vírus (DENV) vector ranked 3th in the number of cases and inicdence. This study aimed to capture of Aedes aegypti to verify the circulation of DENV serotypes in this city. Entomological surveys were carried in urban area in nine collection points, covering of the city and using Adultrap® traps. After capture all the mosquitoes were identification, and the females were used to detect the DENV with later serotyping by RT-Nested-PCR. Larvae also were captured in many places of city and analyzed by similar technique. A total of 18,541 adults mosquitoes were captured, where 6,856 (36.98%) were identified as A, aegypti, with an average female density of 5.74 and prevalence of DENV1, DENV2, DENV4 and DENV3 respectivaley. A total of 1,173 larvae were collected, of which 348 were of the species A. aegypti (49.5%). The DENV detection showed seven positive pools (53.84%) with prevalence of similar serotypes found in adults mosquitoes. Tres Lagoas, have a high infestation of A. aegypti with high DENV positivity with circulation of all four DENV serotypes, corroborating the high number of human dengue cases occurs anually.
