Cell therapy with adipose tissue mesenchymal stem cells improves the quality of the germinal epithelium in swiss mice
International Journal of Development Research
Cell therapy with adipose tissue mesenchymal stem cells improves the quality of the germinal epithelium in swiss mice
Received 13th December, 2018; Received in revised form 29th January, 2019; Accepted 20th February, 2019; Published online 29th March, 2019
Copyright © 2019, Juliano Rodrigues de Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cancer is a disease that affects a large number of people and these are usually undergoing chemotherapy. One of the adverse effects of this therapy is testicular lesions with reduced fertility and one of the alternatives for the treatment of these cases is cell therapy. Thus, the present study evaluated a protocol for explant of mesenchymal stem cells from adipose tissue as well as evaluated the effects of cell therapy with mesenchymal stem cells from adipose tissue under the germinal epithelium of Swiss mice treated with cyclophosphamide. The experimental groups were: (I) Negative Control (NC) - the animals were treated with injection water; Positive Control (PC) - animals were treated with cyclophosphamide at a dose of 150 mg / kg; and positive control + mesenchymal stem cells (PC + MSC) - the animals were treated with cyclophosphamide at the dose of 150 mg / kg and transplanted with 1.0x106 mesenchymal stem cells. The results indicated that transplantation of MSC does not alter the frequency of micronuclei in the peripheral blood. However, it improves the quality of the germinal epithelium and the quality score of the PC + MSC group is equal to that of the NC group.
