Analytical method development of abamectin + imidaclopridby r-hplc

Abamectin is a natural product being used as an insecticide, which has the power of killing the worms, it is also being used as a plant parasitic nematodes. Imidacloprid is a chemical which has the property of neurotoxin to insects. by affecting the Central Nerve System (CNS) of insect and this causes the growth of the insect. The concentrations of these two molecules as a pesticide consider as an important combination against insecticide in the pest management programs. A simple HPLC chromatographic method has been developed and subsequently validated for the combination pesticide (Abamectin + Imidacloprid) separation and quantification. TheseAbamectin + Imidaclopridmoleculeswere separatedthrough a mobile phase consisting of the mixture of acetonitrile and water (fortified with 0.1% of Ortho Phosphoric Acid) in the ratio of 80:20 v/v. The separation was achieved through theQualisil BDS C18 (250 x 4, 5μ) column with the flow rate as 1.0 ml/min with the detection at 230 nm. These method parameters were loaded in the Shimadzu HPLC (model: LC-2030). The LC solution Shimadzu software was used for all the calculations in this analytical method validation analysis. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of these moleculesinterims of validation parameters viz., separation, system suitability, System Precision and linearity in a simple HPLC analysis.