Analysis of immunohistochemical expression of pecam-1 and endoglin in normal skin, benign melanocytic nevi, and cutaneous malignant melanoma
International Journal of Development Research
Analysis of immunohistochemical expression of pecam-1 and endoglin in normal skin, benign melanocytic nevi, and cutaneous malignant melanoma
Received 10th August, 2021 Received in revised form 27th September, 2021 Accepted 19th October, 2021 Published online 28th November, 2021
Copyright © 2021, Marcos V. M. Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objetive: We analyzed expression of PECAM-1 and endoglin for a correlation with clinicopathological behavior in CMM. Methods: Control (n = 12), CMN (n = 48), and CMM (n = 44) samples were submitted for immunohistochemistry. PECAM-1 and endoglin expression were counted in the stroma (hot spots) of all samples in order to calculate the MVD. Data analyses were performed using univariate statistical tests, with significance set at p<0.05. Results: Our findings showed that CMM exhibited higher MVD estimates for both PECAM-1 and endoglin compared to control and CMN samples (p<0.001, for all associations). Moreover, CMN samples exhibited higher MVD compared to control samples (p<0.001 for all associations). CMM from subjects with metastatic disease showed higher MVD by PECAM-1 (p=0.036) and endoglin (p=0.015) compared to non-metastatic CMM. Conclusion: Increasing MVD from normal skin to benign and malignant melanocytic tumors suggests the importance of a rich vascular network in the peritumoral stroma to support greater metabolic and energetic demands, which favors the dissemination of melanocytic tumor cells.
