Statistical approach of the physicalchemical investigation of chitosan/collagen biomatrix crosslinked with gradient of genipin for tissue engineering

Introduction: More than 60 million people in the United States have had an increase in their life expectancy in recent years because of artificial tissue and organ therapy, with every five people over 65 years old. In Brazil, this figure was higher. Thus, tissue engineering contemplates numerous advantages that meet the needs of the injured tissue or organ for the regeneration process. Objective: to investigate the physicochemical properties of chitosan/collagen cross linked with a gradient of genipin for tissue engineering. Methods: Chitosan/collagen biomatrices were prepared and crosslinked with a gradient of genipin for swelling, degradation and cross linking degree investigations, with statistical approach. Results: 0.75 % v/v genomic composite biomatrix indicated to present the best physicochemical characteristics for future clinical studies. Conclusion: With the increase of the degree of interlocking with genipin, the biomatrices became more rigid, reducing the degree of swelling and of free amino groups. Thus, through the present investigation, the chitosan/collagen biomatrix with 0.75 % v/v genipin provided the best physicochemical properties for future cell and clinical studies.