Polymorphism of microsatellite markers developed from homologous genomic regions of phaseolus vulgaris to meloidogyne ssp. resistance genes identified in soybean
International Journal of Development Research
Polymorphism of microsatellite markers developed from homologous genomic regions of phaseolus vulgaris to meloidogyne ssp. resistance genes identified in soybean
Received 19th February, 2020; Received in revised form 17th March, 2020; Accepted 29th April, 2020; Published online 25th May, 2020
Copyright © 2020, Letícia de Maria O. Mendes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DNA markers can contribute to the genetic control selection of many diseases in plants. Nematodes belonging to genus Meloidogyne gather pathogens that cause damage and yield losses in Phaseolus vulgaris. Although the sources of genetic resistance variations are known in legumes such as soybeans, little is known about this feature in common bean crops. This study aimed to characterize the polymorphism of microsatellite markers developed to extend the resistance genes of P. vulgaris to Meloidogyne spp. Twenty-six cultivars were used to characterize nine microsatellite markers that have developed from homologous genomic regions of P. vulgaris to six genes identified in soybean (EREBP; Ext1; Ext2; Rhg1; Rhg4; Rmi1), which are associated with resistance to root-knot nematodes. Six of the nine microsatellite primers were polymorphic. In total, 12 alleles were produced - two alleles per amplified microsatellite locus. The mean gene diversity was 0.36; no sample presented heterozygosity and this outcome corroborates the fixation index equal to 1.00. Probability of identity and of exclusion multiloci were 0.01 and 0.78, respectively. Despite the reduced ability of microsatellite markers to detect polymorphism, they are the first DNA markers developed to extend the resistance genes of common beans to Meloidogyne ssp. available for validation studies.