Detection of encoding gene extended spectrum beta lactamase on escherichia coli isolated from broiler chicken meat in traditional market surabaya
International Journal of Development Research
Detection of encoding gene extended spectrum beta lactamase on escherichia coli isolated from broiler chicken meat in traditional market surabaya
Received 22nd August 2017; Received in revised form 07th September, 2017; Accepted 16th October, 2017; Published online 30th November, 2017
Copyright ©2017, Rinda Dewi Safitri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study aims to isolate, to identify, and to seek out fragments of encoding gene Extended Spectrum β-Lactamase on Escherichia coli isolated from swab surface of broiler chicken meat in a number of traditional markets in Surabaya. The result shows that 31 out of 50 samples positively contain Escherichia coli, shown through EMBA isolation media and identified using indole test. Sensitivity test shows that 100% of the isolates are resistant to Ampicilin, 48.4% are resistant to Cephazoline, 13% are resistant to Ceftazidime, 9.6% are resistant to Cefotaxime, 6.4% are resistant to Ceftriaxone and 87.2% are resistant to Tetracycline. 8 out of 8 (100%) samples of E. coli resistant show the presence of band towards blaTEM gene of 768 basepair (bp).
