Invitro anticancer activity of coconut shell oil on breast cancer cell line
International Journal of Development Research
Invitro anticancer activity of coconut shell oil on breast cancer cell line
Received 11th December, 2017; Received in revised form 06th January, 2018; Accepted 05th February, 2018; Published online 30th March, 2018
Copyright © 2018, Dorathy Selva Jeba Pritha and Karpagam. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The ethanolic extract of Coconut shell oil were analysed for the anticancer activity against breast cancer cell lines. Coconut shells were collected and further processed for extract preparation. Breast cancer cell line was obtained from NCCS, Pune and was maintained in DMEM High Glucose media in 10% FBS, Penecillin (100 U/ml) and Streptomycin (100 µg/ml).The antiproliferative activity of Coconut shell oil were analysed by 0.5% 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) method. The highest non toxic concentration was observed at 125µg/ml concentration of ethanol extract of Coconut shell oil recorded in the cell line. These results showed the potential of Coconut shell oil as anticancer activity and further studies are necessary to analyse their mechanism and to isolate their anticancer compounds that is responsible for such activity.
