Antitumoral activity of eleutherine plicata herb. and its compounds
International Journal of Development Research
Antitumoral activity of eleutherine plicata herb. and its compounds
Received 09th December, 2020; Received in revised form 16th December, 2020; Accepted 06th January, 2021; Published online 28th February, 2021
Copyright © 2020, Ana Laura Gadelha Castro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The antitumoral activity of plants containing quinonic compounds has been reported, however there is a need to evaluate Eleutherine plicata. This study assesses the impact of E. plicata fractionation in its antitumoral effect. The ethanol extract obtained from E. plicata bulbs (EEEP) was fractionated under reflux. The dichloromethane fraction (FRDCM) was refracted in a chromatographic column, isolating eleutherol, isoeleutherin and eleutherin. The antitumoralactivity was evaluated in cell line (oral cancer-SCC-9) and normal cell line (human keratinocytes-HaCaT) using the sulforadamine, three-dimensional spheroid and cell migration assays. The greatest inhibitions of cell proliferation and selectivity index were observed with EEEP (SCC-9: IC50 = 12.87 ± 0.86 and HaCaT: 28.81 ± 1.82 µg/mL). Cell desagregation was influenced by the exposure time and concentration of EEEP. Greater inhibitory effect on speed and targeting was observed in tumor cells treated with EEEP. In summary, EEEP has a promisingantitumor activity.
